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Table 1 Age- and ovarian hormone-related changes in expression of genes related to microglial reactivity in the frontal cortex of middle-aged female rats

From: Menopause leads to elevated expression of macrophage-associated genes in the aging frontal cortex: rat and human studies identify strikingly similar changes

Symbol

Taqman assay ID

RQ (age)

P (age)

RQ (OVX)

P (OVX)

RQ (age+OVX)

P (age+OVX)

Macrophage-associated genes

Phagocytic and scavenger receptors

Cd11b b

Rn00709342_m1

1.389 a

0.044

2.000 a

< 0.001

2.778 a

< 0.001

Cd18 b

Rn01427948_m1

1.206

 

1.488 a

0.001

2.164 a

< 0.001

Cd93

Rn00584525_g1

1.271

0.082

0.796

0.051

1.012

 

Fcgr2b

Rn00598391_m1

1.560 a

0.024

1.595 a

< 0.001

2.488 a

< 0.001

Msr2

Rn01455191_m1

2.630

 

1.353 a

0.025

3.558 a

0.003

Cd36

Rn00580728_m1

0.567 a

<0.001

0.776 a

0.012

0.440 a

< 0.001

Rage

Rn00584249_m1

1.225

 

0.870

 

1.066

 

Recognition receptors

Cd14

Rn00572656_g1

0.844

 

1.391

 

1.174

 

Cd40 b

Rn01423590_m1

1.496

0.087

0.990

 

1.481

0.054

Cd45 b

Rn00709901_m1

1.196

 

1.504 a

< 0.001

1.799 a

< 0.001

Cd86 b

Rn00571654_m1

1.178

 

1.513 a

0.001

1.782 a

< 0.001

Tlr2

Rn02133647_s1

1.015

 

1.000

 

1.015

 

Tlr4

Rn00569848_m1

1.207

 

1.253 a

0.006

1.512 a

< 0.001

Tlr9

Rn01640054_m1

1.452 a

0.033

1.348

 

1.907 a

0.008

RT1-Aw2

Rn03034964_u1

14.696 a

< 0.001

1.479 a

0.001

21.735 a

< 0.001

RT1-N1

Rn00561858_m1

0.588 a

0.046

3.300 a

< 0.001

1.941 a

< 0.001

Cd74

Rn00565062_m1

2.379 a

0.031

0.948

 

2.255 a

0.047

Signaling

Nos2

Rn00561646_m1

0.971

 

1.072

 

1.041

 

Irf7

Rn01450778_g1

1.855

 

2.519 a

< 0.001

4.748 a

< 0.001

Irf9

Rn01489163_m1

1.332 a

0.030

1.311 a

0.004

1.746 a

< 0.001

Regulatory genes for microglia reactivity

Microglial receptors

Cd47

Rn00569914_m1

0.884

0.084

1.189 a

0.041

1.051

 

Cd200r

Rn00576646_m1

0.821 a

0.005

0.880

0.069

0.722 a

< 0.001

Cx3cr1

Rn00591798_m1

1.375 a

0.006

0.975

 

1.341 a

0.005

Neuronal ligands

Cd200

Rn00580478_m1

0.945

 

0.878

0.088

0.829 a

0.019

Sirpa

Rn00564609_m1

0.922

 

1.071

 

0.987

 

Cx3cl1

Rn00593186_m1

0.928

 

0.813 a

< 0.001

0.754 a

< 0.001

Complement and cytokine genes

Complement

C1qb

Rn00570480_m1

1.739 a

< 0.001

0.951

 

1.654 a

< 0.001

C1-Inh

Rn01485600_m1

1.008

 

1.541 a

0.005

1.553 a

0.009

C3

Rn00566466_m1

4.297 a

< 0.001

1.193 a

0.010

5.126 a

< 0.001

Chemokine and cytokines

Ccl2

Rn00580555_m1

1.294

 

1.634 a

0.079

2.114 a

0.014

Il1b

Rn00580432_m1

2.390 a

< 0.001

0.518 a

<0.001

1.238

 

Il6

Rn00561420_m1

1.119

 

0.651

 

0.728

 

Tgfb1

Rn00572010_m1

0.898

0.057

1.500 a

< 0.001

1.347 a

< 0.001

Tnf

Rn99999017_m1

0.843

 

1.595

0.084

1.345

 
  1. Quiescent and activated microglial cells differ strikingly in the expression of macrophage-associated genes. Age- and ovariectomy-related changes in mRNA expression were measured by real-time PCR. The applied TaqMan assays were listed. RQ represents the expression of a given gene in response to aging or ovariectomy compared to basal (control) expression. RQ (age) represents the ratio of mRNA expression in middle-aged versus young rats. RQ (OVX) shows the ratio of mRNA levels in middle-aged OVX rats versus middle-aged ones. RQ (age+OVX) values correspond to the ratio of mRNA expression in middle-aged OVX rats compared to young ones. Statistical significance of the alterations was tested by analysis of variance (ANOVA) with Newman-Keuls post hoc test, and was considered significant at P < 0.05; aRQ values with statistically significant changes. P-values are indicated if < 0.1. bMicroglial marker genes.
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Journal of Neuroinflammation

ISSN: 1742-2094