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Table 1 Age- and ovarian hormone-related changes in expression of genes related to microglial reactivity in the frontal cortex of middle-aged female rats

From: Menopause leads to elevated expression of macrophage-associated genes in the aging frontal cortex: rat and human studies identify strikingly similar changes

Symbol Taqman assay ID RQ (age) P (age) RQ (OVX) P (OVX) RQ (age+OVX) P (age+OVX)
Macrophage-associated genes
Phagocytic and scavenger receptors
Cd11b b Rn00709342_m1 1.389 a 0.044 2.000 a < 0.001 2.778 a < 0.001
Cd18 b Rn01427948_m1 1.206   1.488 a 0.001 2.164 a < 0.001
Cd93 Rn00584525_g1 1.271 0.082 0.796 0.051 1.012  
Fcgr2b Rn00598391_m1 1.560 a 0.024 1.595 a < 0.001 2.488 a < 0.001
Msr2 Rn01455191_m1 2.630   1.353 a 0.025 3.558 a 0.003
Cd36 Rn00580728_m1 0.567 a <0.001 0.776 a 0.012 0.440 a < 0.001
Rage Rn00584249_m1 1.225   0.870   1.066  
Recognition receptors
Cd14 Rn00572656_g1 0.844   1.391   1.174  
Cd40 b Rn01423590_m1 1.496 0.087 0.990   1.481 0.054
Cd45 b Rn00709901_m1 1.196   1.504 a < 0.001 1.799 a < 0.001
Cd86 b Rn00571654_m1 1.178   1.513 a 0.001 1.782 a < 0.001
Tlr2 Rn02133647_s1 1.015   1.000   1.015  
Tlr4 Rn00569848_m1 1.207   1.253 a 0.006 1.512 a < 0.001
Tlr9 Rn01640054_m1 1.452 a 0.033 1.348   1.907 a 0.008
RT1-Aw2 Rn03034964_u1 14.696 a < 0.001 1.479 a 0.001 21.735 a < 0.001
RT1-N1 Rn00561858_m1 0.588 a 0.046 3.300 a < 0.001 1.941 a < 0.001
Cd74 Rn00565062_m1 2.379 a 0.031 0.948   2.255 a 0.047
Signaling
Nos2 Rn00561646_m1 0.971   1.072   1.041  
Irf7 Rn01450778_g1 1.855   2.519 a < 0.001 4.748 a < 0.001
Irf9 Rn01489163_m1 1.332 a 0.030 1.311 a 0.004 1.746 a < 0.001
Regulatory genes for microglia reactivity
Microglial receptors
Cd47 Rn00569914_m1 0.884 0.084 1.189 a 0.041 1.051  
Cd200r Rn00576646_m1 0.821 a 0.005 0.880 0.069 0.722 a < 0.001
Cx3cr1 Rn00591798_m1 1.375 a 0.006 0.975   1.341 a 0.005
Neuronal ligands
Cd200 Rn00580478_m1 0.945   0.878 0.088 0.829 a 0.019
Sirpa Rn00564609_m1 0.922   1.071   0.987  
Cx3cl1 Rn00593186_m1 0.928   0.813 a < 0.001 0.754 a < 0.001
Complement and cytokine genes
Complement
C1qb Rn00570480_m1 1.739 a < 0.001 0.951   1.654 a < 0.001
C1-Inh Rn01485600_m1 1.008   1.541 a 0.005 1.553 a 0.009
C3 Rn00566466_m1 4.297 a < 0.001 1.193 a 0.010 5.126 a < 0.001
Chemokine and cytokines
Ccl2 Rn00580555_m1 1.294   1.634 a 0.079 2.114 a 0.014
Il1b Rn00580432_m1 2.390 a < 0.001 0.518 a <0.001 1.238  
Il6 Rn00561420_m1 1.119   0.651   0.728  
Tgfb1 Rn00572010_m1 0.898 0.057 1.500 a < 0.001 1.347 a < 0.001
Tnf Rn99999017_m1 0.843   1.595 0.084 1.345  
  1. Quiescent and activated microglial cells differ strikingly in the expression of macrophage-associated genes. Age- and ovariectomy-related changes in mRNA expression were measured by real-time PCR. The applied TaqMan assays were listed. RQ represents the expression of a given gene in response to aging or ovariectomy compared to basal (control) expression. RQ (age) represents the ratio of mRNA expression in middle-aged versus young rats. RQ (OVX) shows the ratio of mRNA levels in middle-aged OVX rats versus middle-aged ones. RQ (age+OVX) values correspond to the ratio of mRNA expression in middle-aged OVX rats compared to young ones. Statistical significance of the alterations was tested by analysis of variance (ANOVA) with Newman-Keuls post hoc test, and was considered significant at P < 0.05; aRQ values with statistically significant changes. P-values are indicated if < 0.1. bMicroglial marker genes.