Figure 2
Early treatment with AP reduces T-cell proliferation to MOG35-55. C57BL/6 mice were immunized with MOG35-55 in CFA and treated daily with AP from day 0 to 6. Splenocytes were isolated at day 28 and proliferation of 9 individual mice per group was measured by [3H]-thymidine incorporation in response to increasing doses of MOG35-55 (A). The supernatant of these cultures was assessed for the presence of IFN-γ (B), IL-17A (C) and TNF-α (D). In a separate experiment, mice (n = 5 per group) were treated with AP or control diluent from day 0 to 6, splenocytes were harvested on day 10 and proliferation to MOG35-55 was assessed as described above (E). Data indicate mean ± sem; * P <0.05 Mann–Whitney U test. AP, alkaline phosphatase; CFA, complete Freund’s adjuvant; sem, standard error of the mean; TNF-α, tumor necrosis factor α.