Specific knockdown of MIP-2γ expression in cultured astrocytes by siRNA. Representative western blots of MIP-2γ and GFAP. The expression of MIP-2γ was significantly increased after pAAV-MIP-2γ-hrGFP transfection (MIP-2γ) compared with untreated cultures (control) or transfected with pAAV-IRES-hrGFP (mock) vector. This increase in MIP-2γ was inhibited by co-transfection with MIP-2γ siRNAs (MIP-2γ + siRNA-1, MIP-2γ + siRNA-2, and MIP-2γ + siRNA-3), but not by pBS/U6-con (MIP-2γ + BS/U6-con) vector. No significant changes in GFAP occurred. Equal loading was confirmed by exposing the membranes to an anti β-actin antibody. One of four experiments that generated the same result is shown. GFAP, glial fibrillary acid protein; MIP-2γ, macrophage inflammatory protein-2γ; siRNA, small interfering RNA.