Overexpression of MIP-2γ inhibits GLT-1 expression in lipid rafts and increases caveolin-1 expression. Equal amounts of proteins from collected fractions were separated by SDS-PAGE and analyzed by immunoblotting using antibodies against GLT-1, GLAST, flotillin-1, and caveolin-1. Raft domains (R) are recovered in low-density fractions 3, 4 and 5, which are enriched in flotilin-1, a component of lipid rafts. Fractions 8, 9, and 10 correspond to detergent-soluble material (S). Co-fractionation of GLAST and GLT-1 with the lipid raft marker flotillin-1 and caveolin-1 after sucrose density ultracentrifugation. Control, untreated cultures. Mock, astrocytes transfected with pAAV-IRES-hrGFP vector. MIP-2γ, astrocytes transfected with pAAV-MIP-2γ-hrGFP plasmids. GLAST, glutamate-aspartate transporter; GLT-1, glutamate transporter; IgG, immunoglobulin G; MIP-2γ, macrophage inflammatory protein-2γ.