Effect of pharmacological inhibitors on GLT-1 mRNA and protein expression. GLT-1 mRNA (a) and protein (b) expression in astrocytes either untreated (control) or transfected with pAAV-MIP-2γ-hrGFP plasmids (MIP-2γ) in the absence or presence (+) of PDTC (100 μM), LY294002 (10 μM), KT5720 (5 μM), or PD98059 (50 μM) for 48 hours. Individual experiments were performed three times with triplicate samples. Results were expressed as fold of corresponding mean ± SEM after normalization to β-actin. *P <0.05; **P <0.01 compared with control group; §
P <0.05; §§
P <0.01 compared with MIP-2γ group; one-way ANOVA, Tukey test. ANOVA, analysis of variance; GLT-1, glutamate transporter; MIP-2γ, macrophage inflammatory protein-2γ; SEM, standard error of the mean.