Figure 9

Central nervous system pathology in CD4-depleted mice. (A) Spinal cords at day 10 post-infection (p.i). stained with Luxol fast blue LFB (to identify areas of myelin loss) in wild-type (WT) and B7-H1^−/− mice show similar extent of demyelination after treatment with α-CD4 or control α-βgalactosidase (α-βgal) monoclonal antibody (mAb). Magnification bar = 100 μm. (B) Axonal integrity within demyelinated lesions visualized with α-SMI-31 plus α-SMI-32 Ab. Demyelinating lesions showed more extensive axonal damage in B7-H1^−/− mice, with larger areas of axonal loss (dotted outline) and increased numbers of swollen axons (arrows). After α-CD4 mAb treatment, lesions appeared to have reduced axonal loss and decreased numbers of swollen axons. Magnification bar = 100 μm.