PINK1 potentiates NF-κB activity via Tollip suppression in response to IL-1β.
−/− and PINK1
+/+ mouse embryo fibroblasts were transfected for 36 hours with the NF-κB-responsive luciferase reporter and control renilla luciferase reporter alone or together with HA-Tollip and Myc-hPINK1-WT, as indicated. After cells were treated for 12 hourswith 50 ng/ml IL-1β, relative luciferase activity was measured and normalized to renilla activity. Error bars indicate ± standard deviation in triplicate experiments.***P<0.001. PINK1, PTEN-induced putative kinase 1.