Effect of endothelin-1 on glial activation in adult and juvenile brains. Representative immunohistochemical staining of glial fibrillary acidic protein (GFAP) in the juvenile (A) and adult (C) brains cortex and quantitative analysis of GFAP expression (E). Comparisons between groups were performed using one-way analysis of variance (ANOVA) with Bonferroni post test. Data are presented as mean ± S.E.M. Statistical significance was set at P <0.05. Each bar is expressed as the mean ± S.E.M; ***P <0 .0001 compared with control group. (D) Representative immunohistochemical staining of GFAP in the juvenile brain and adult (F) hippocampus and quantitative analysis of GFAP expression (E). Each bar is expressed as the mean ± S.E.M; ***P <0.0001 compared with control group.