Cytokines and chemokines levels for 24 hours in juveniles (P21) and adult (P84) rat brains after a single intracerebral injection of endothelin-1. All cytokines, IL-1α, IL-1β, IL-6, IL-10, MCP-1/ccl2, fractalkine and TIMP-1 were simultaneously measured in brain and serum samples using the Agilent technology cytokine microarray (Tebu-bio). All brain values were corrected for individual serum measurements as described previously . Comparisons between groups were performed using one-way analysis of variance (ANOVA) with the Student-Bonferroni post test. Data are presented as mean ± S.E.M. Statistical significance was set at P <0.05. Graph plotted as mean ± S.E.M. *P <0.05, **P <0.01, ***P < 0.0001 compared with animal controls. The number of animal tested: n = 3 or 5 rats for each group.