6 immunoreactivity in the walls of the middle cerebral artery
and microvessels following subarachnoid hemorrhage
. Images represent (A) sham, SAH + vehicle and SAH + U0126, 2 days after SAH; (B) sham, SAH + vehicle and SAH + U0126, 3 days after SAH; and (C) sham and SAH, 4 days after SAH. There was a significant increase in IL-6 protein levels in the wall of the MCA and surrounding microvessels (white arrows) after SAH + vehicle as compared to sham-operated rats at 2 and 3 days with maximum at 3 days. IL-6 immunoreactivity was slightly increased in MCA at 4 days in the SAH group as compared to the sham group. Treatment with U0126 given at 6 h after induced SAH prevented the increased protein expression in both MCA and microvessels at 2 and 3 days. IL-6 was located in both SMCs and in the endothelium (arrowhead). (D) Bar graph showing semiquantification of fluorescence intensity for IL-6 in MCA. Data are presented as the mean percentage relative to sham ± SEM; n = 5. *P <0.05, **P <0.01.