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Table 1 Summary of experimental groups

From: Regulation of enhanced cerebrovascular expression of proinflammatory mediators in experimental subarachnoid hemorrhage via the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase pathway

Experimental group

N

Time after SAH

  

2 min

1 h

6 h

12 h

24 h

36 h

48 h

72 h

96 h

Immunohistochemistry: temporal profile of proinflammatory mediators, pERK1/2 and effect of U0126

0 h (2 min SAH)

3

X

        

1 h SAH

3

 

X

       

6 h SAH

3

  

X

      

12 h SAH

3

   

X

     

24 h SAH

3

    

X

    

48 h sham

6

      

X

  

48 h SAH + vehicle

5

  

V

V

V

V

X

  

48 h SAH + U0126

6

  

U

U

U

U

X

  

72 h sham

5

       

X

 

72 h SAH + vehicle

6

  

V

V

V

  

X

 

72 h SAH + U0126

6

  

U

U

U

  

X

 

96 h sham

7

        

X

96 h SAH

6

        

X

Western blots: ERK activation at early timepoints after SAH

1 h sham

3

 

X

       

1 h SAH

3

 

X

       

6 h sham

3

  

X

      

6 h SAH

3

  

X

      

24 h sham

3

    

X

    

24 h SAH + vehicle

3

  

V

V

X

    

24 h SAH + U0126

3

  

U

U

X

    
  1. X indicates termination of all rats in each group. V indicates treatment with vehicle (0.01% dimethylsulfoxide) and U indicates treatment with U0126.
  2. ERK = extracellular signal-regulated kinase; N = no.of rats in each group; SAH = subarachnoid hemorrhage.