Experimental group | N | Time after SAH | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
 |  | 2 min | 1 h | 6 h | 12 h | 24 h | 36 h | 48 h | 72 h | 96 h |
Immunohistochemistry: temporal profile of proinflammatory mediators, pERK1/2 and effect of U0126 | ||||||||||
0 h (2 min SAH) | 3 | X | Â | Â | Â | Â | Â | Â | Â | Â |
1 h SAH | 3 | Â | X | Â | Â | Â | Â | Â | Â | Â |
6 h SAH | 3 | Â | Â | X | Â | Â | Â | Â | Â | Â |
12 h SAH | 3 | Â | Â | Â | X | Â | Â | Â | Â | Â |
24 h SAH | 3 | Â | Â | Â | Â | X | Â | Â | Â | Â |
48 h sham | 6 | Â | Â | Â | Â | Â | Â | X | Â | Â |
48 h SAH + vehicle | 5 |  |  | V | V | V | V | X |  |  |
48 h SAH + U0126 | 6 |  |  | U | U | U | U | X |  |  |
72 h sham | 5 | Â | Â | Â | Â | Â | Â | Â | X | Â |
72 h SAH + vehicle | 6 |  |  | V | V | V |  |  | X |  |
72 h SAH + U0126 | 6 |  |  | U | U | U |  |  | X |  |
96 h sham | 7 | Â | Â | Â | Â | Â | Â | Â | Â | X |
96 h SAH | 6 | Â | Â | Â | Â | Â | Â | Â | Â | X |
Western blots: ERK activation at early timepoints after SAH | ||||||||||
1 h sham | 3 | Â | X | Â | Â | Â | Â | Â | Â | Â |
1 h SAH | 3 | Â | X | Â | Â | Â | Â | Â | Â | Â |
6 h sham | 3 | Â | Â | X | Â | Â | Â | Â | Â | Â |
6 h SAH | 3 | Â | Â | X | Â | Â | Â | Â | Â | Â |
24 h sham | 3 | Â | Â | Â | Â | X | Â | Â | Â | Â |
24 h SAH + vehicle | 3 |  |  | V | V | X |  |  |  |  |
24 h SAH + U0126 | 3 |  |  | U | U | X |  |  |  |  |