Transwell carrying activated microglia, but not the conditioned medium, decreased L-Glu uptake in the astrocyte culture. (A) Effect of the conditioned medium collected from the astrocyte-microglia-neuron mixed culture treated with LPS (10 ng/mL, 72 h) on L-Glu clearance in the astrocyte culture. A 72-h incubation with the conditioned medium did not affect L-Glu clearance in the astrocyte culture. (B) The effects of transwell-cultured microglia in the presence of LPS (10 ng/mL, 72 h) on L-Glu uptake in the astrocyte culture. A significant decrease in the L-Glu uptake was caused by 72-h treatment with the transwell. **P < 0.01 vs. control group (n = 6), Student’s t-test.