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Figure 7 | Journal of Neuroinflammation

Figure 7

From: L-glutamate released from activated microglia downregulates astrocytic L-glutamate transporter expression in neuroinflammation: the ‘collusion’ hypothesis for increased extracellular L-glutamate concentration in neuroinflammation

Figure 7

CBX ameliorated the decrease in L-Glu uptake and GLAST expression in inflammation without cell death. (A) The effect of CBX on L-Glu release from activated microglia. CBX concentration-dependently suppressed L-Glu release due to LPS (10 ng/mL, 24 h)-activated microglia. **P < 0.01 vs. control group, #P < 0.05, ##P < 0.01 vs. the LPS-treated group (n = 5), Tukey’s test following ANOVA. (B) The effects of CBX on the decreased L-Glu uptake in the model of inflammation without cell death. CBX concentration-dependently inhibited the LPS (10 ng/mL, 72 h)-induced decrease in L-Glu uptake in the astrocyte-microglia-neuron mixed culture (left) but not in the astrocyte culture (right). **P < 0.01 vs. control group, #P < 0.05, ##P <0.01 vs. the LPS-treated group (n = 5), Tukey’s test following ANOVA. (C) The effect of CBX on the LPS-induced decrease in GLAST mRNA levels. In the presence of CBX (100 μM), the LPS (10 ng/mL, 24 h)-induced decrease in GLAST mRNA level was ameliorated in the astrocyte-microglia-neuron mixed culture. **P < 0.01 vs. control group, #P < 0.05 vs. the LPS-treated group (n = 5), Tukey’s test following ANOVA. (D) The effect of CBX on the LPS-induced decrease in GLAST protein levels. In the presence of CBX (100 μM), the LPS (10 ng/mL, 72 h)-induced decrease in GLAST protein levels was ameliorated in the astrocyte-microglia-neuron mixed culture. *P < 0.05 vs. control group (n = 4), Tukey’s test following ANOVA.

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