CNS proglucagon mRNA expression in the frontal cortex is reduced in an obese insulin-resistant mouse model. A. Proglucagon mRNA expression in obese ob/ob mice as compared to C57 black littermates. Data are from two groups of adult mice, in total 6 C57 black mice and 9 ob/ob mice, 6-9 months of age. B. TNFα mRNA expression in obese ob/ob mice as compared to C57 black littermates. Data from the same animals as in A. Proglucagon and TNFα mRNA expression was normalized with mRNA expression of GAPDH, which was used as an internal control. Bars represent mean ± SEM. *P < 0.05 for a chance difference. Statistical analysis was performed using Mann-Whitney rank sum test. C. Correlation between proglucagon mRNA and TNFα mRNA. Data from three C57 black mice and five ob/ob mice, 6 months of age; qPCR run in duplicate at two independent occasions. A.u.; arbitrary units.