(A) Immunofluorescence analysis of colonic tissues showing the effect of pentamidine on macrophage infiltration induced by DSS: pentamidine dose-dependently inhibited macrophage infiltration (MAC387, red) and increased p53 expression (green) in colon criptae. Arrows indicate MAC387/p53 co-expression; (B) relative quantification (MAC387+/p53 + area/total area percentage) of macrophage infiltration and p53 expression in DSS-treated mice. Data are expressed as mean ± SEM of n = 3 experiments. Original magnification 200X. DSS, dextran sodium sulphate.