Kir4.1 and IL-1β expression in rat temporal cortex after status epilepticus (SE). (A, B) Quantitative real-time PCR. mRNA expression levels of Kir4.1 (A) and IL-1β (B) in the temporal cortex of control rats (n = 6; Con), rats at 1 week (n = 6), and 3 months (n = 6) after SE. Data represent the target gene expression normalized to the reference genes. The error bars represent SEM and * represents a P value <0.05. (C, D) Western blot analysis of Kir4.1. (C) Representative immunoblot of total homogenates from temporal cortex of controls and post SE (24 h and 1 week) rats. (D) Densitometric analysis: values (optical density units, O.D.) are mean ± SEM, (control, n = 5; 24 h post SE, n = 5; and 1 week post SE, n = 5), relative to the optical density of β-tubulin; *P <0.05, compared to controls.