Kir4.1 (IR) in U373 glioblastoma cell line and in cultured human astrocytes after exposure to IL-1β. (A-D) representative photomicrographs showing Kir4.1 IR in glioma cells (U373; A, B) and cultured human astrocytes (C, D), untreated (A and C; Con) and treated (B and D) for 24 h with 10 ng/mL IL-1β; high magnifications are shown in the inserts; in U373 cells Kir4.1 was also detected in the nuclei of glial cells (insert in A and B). Scale bar in A: A and B: 80 μm; C and D: 40 μm. (E and F): percentage of Kir4.1 positivity in glioma cells (U373; E) and cultured human astrocytes (F) untreated and treated for 24 h with 10 ng/mL IL-1β. *P <0.05, compared to untreated controls (Con).