HLA-DR and HMGB1 expression in glial tumors from patients with and without epilepsy. (A-D) Representative photomicrographs of HLA-DR IR in astrocytoma grade III (A III; A and B) and glioblastoma multiforme (GBM; C and D) with (A and C) and without (B and D) epilepsy, showing immmunoreactive cells in epilepsy associated tumors. (E-H) Representative photomicrographs of HMGB1 IR in A III (E and F) and GBM (G and H) with (E and G) and without (F and H) epilepsy, showing cytoplasmic staining (arrows in E and G) in epilepsy-associated tumors. Sections were counterstained with hematoxylin. Scale bars: A-C: 160 μm; E-H: 60 μm. (I) Quantification bargrams of HMGB1-positive cells in tumors with and without epilepsy. Extranuclear staining in epilepsy associated tumors: *P <0.05 vs. tumors without epilepsy.