Histopathology of PLP-induced EAE in HLA DQB1*0602 Tg mouse. Samples were taken on day19 after immunization. Panel (A) shows the spinal cord, (B) the cerebellum and (C) the optic nerve. The sections were stained with hematoxylin & eosin (a), Luxol fast blue for myelin. (b) Bieschowsky silver impregnation for axons (c) and by immunocytochemistry for CD3 (d), and Mac-3 (e). Note the unusually profound inflammation in the cortex and optic nerve. Original magnifications: A and B: × 25; C: × 100.