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Table 2 Active EAE induction with hPLP peptides

From: DQB1*0602 rather than DRB1*1501 confers susceptibility to multiple sclerosis-like disease induced by proteolipid protein (PLP)

HLA Tg mice

Immunization

Incidence

Maximal clinical severity

Day of onset

DRB1*1501

hPLP30-51

0/4

--

--

 

hPLP30-51*

0/4

--

--

 

hPLP41-60

0/4

--

--

 

hPLP41-60*

0/4

--

--

 

hPLP95-116*

0/4

--

--

 

hPLP139-151*

0/3

--

--

 

hPLP175-194

0/3

--

--

 

hPLP175-194*

0/4

--

--

 

hPLP185-206

0/3

--

--

 

hPLP185-206*

0/9

--

--

 

hPLP195-216

0/3

--

--

 

hPLP195-216*

0/4

--

--

 

hPLP206-226

0/3

--

--

 

hPLP206-226*

0/3

--

--

 

hPLP257-276*

0/3

--

--

 

hΔPLP*

0/4

--

--

DQB1*0602

hPLP30-51

0/2

--

--

 

hPLP30-51*

0/4

--

--

 

hPLP84-102

0/2

--

--

 

hPLP139-151*

6/15

1,2,1,1,1,1

13,15,16,13,14,14

 

hPLP175-194*

5/9

2,3,1,3,1

13,14,15,20, 21

 

h175-183S-194*

0/5

--

--

 

hPLP206-226

0/3

--

--

 

hPLP206-226*

0/4

--

--

 

hPLP215-235

0/4

--

--

 

hPLP215-235*

0/4

--

--

 

hPLP257-276

0/8

--

--

 

hPLP257-276*

0/4

--

--

 

hΔPLP*, **

0/8

--

--

  1. Mice were injected with 200 μg of PLP peptide or hΔPLP, and received 300 ng PT immediately and after 48 h (protocol 1)
  2. *, Mice received a boost after a week (protocol 2)
  3. **, The Cys to Ser replacements in the hΔPLP abrogated the encephalitogeinc potential of epitopes, as shown here for PLP175-183Cys/Ser-194 mutated peptide and detailed in Results