Histologic changes and TRPV1 immunoreactivity in the distal colon and the urinary bladder during colonic inflammation. TNBS treatment resulted in inflammatory response of the distal colon (A-B) and increased immunoreactivity of TRPV1 in the distal colon (E-F). Colonic inflammation was not accompanied by bladder inflammation (C-D), or changes in the TRPV1 immunoreactivity in the urinary bladder (G-H). Four independent experiments showed consistent results. Quantification of the results were shown in I and J. Bar = 300 μm in A-D; Bar = 50 μm in E-H. *, p < 0.05.