Effects of BDNF antibody and RTX on the urinary bladder activity during colonic inflammation. Spinal intrathecal BDNF neutralizing antibody reversed the colonic inflammation-induced decreases in micturition intervals by 75% (A), and also reversed the decreased voiding amount measured by a scale (B). Pre-treatment with RTX reversed the decreased micturition intervals to normal level (C), and also reversed the decreases in voiding quantity caused by colonic inflammation. n = 3 to 5 animals for each treatment. *, p < 0.05 vs vehicle or control IgG; #, p < 0.05 vs TNBS + vehicle or TNBS + IgG.