Figure 10

Summary of the potential mechanism of NOX2 inhibition by naloxone. Naloxone (Nal) inhibits activation of NOX2 and superoxide production by directly binding to the gp91^phox-p22^phox complex, and then preventing the translocation of cytosolic subunits. It is interesting to note that the binding capacity of ³H-naloxone to the gp91^phox-p22^phox complex was greatly enhanced after activation of NOX2. Binding of naloxone to the gp91^phox-p22^phox complex may change the conformation of the protein complex and subsequently decrease the affinity of binding of the cytosolic subunits (p47^phox, p67^phox).