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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Naloxone inhibits immune cell function by suppressing superoxide production through a direct interaction with gp91phoxsubunit of NADPH oxidase

Figure 2

Displacement of 3 H-naloxone by both (-) and (+)-naloxone in BV2 microglia. BV2 microglia (2 × 106) were used for binding experiments. Cells were incubated with hot and indicated fold concentrations of cold naloxone in binding buffer (HBSS + 10% serum) for 2 hours at 4°C. Afterward, the medium was removed and cells or membrane were washed seven times with ice-cold HBSS. Cells were lysed with 400 μl of 1 N NaOH. Cell lysate or membrane was mixed with 10 ml of Ultima Gold scintillation fluid and counted for radioactivity. Results are expressed as percentage of total binding observed with hot naloxone alone and are mean ± SEM of three independent experiments performed in duplicate. **P < 0.01 compared with the corresponding control group, ## P < 0.01 compared with SOD group. HBSS, hank's buffered salt solution.

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