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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Naloxone inhibits immune cell function by suppressing superoxide production through a direct interaction with gp91phoxsubunit of NADPH oxidase

Figure 7

Diphenyliodonium (DPI), a NOX2 inhibitor, reduces 3 H-naloxone binding. A. Neutrophil (WT and gp91phox-/-) and B. COS-7 (WT: transfected with empty vector, gp91phox-p22phox: transfect with gp91phox-p22phox subunit and NOX2: transfected with gp91phox-p22phox and cytosolic subunits, detailed transfection results are shown in Figure 4) were incubated with 3H-naloxone (6 nM) and DPI in binding buffer (HBSS + 10% serum) for 2 hour at 4°C. Afterward, the buffer was removed and cells or membrane were washed seven times with ice-cold HBSS. Then, cells or membranes were mixed with 10 ml of Ultima Gold scintillation fluid and counted for radioactivity. Results are expressed as a percentage of the control and are mean ± SEM of three independent experiments performed in duplicate. **P < 0.01 compared with corresponding control group, ## P < 0.01 compared with NO DPI group.

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