Figure 8

Pre-treatment with naloxone inhibits translocation of the NOX2 p47 ^phox cytosolic subunit to membrane. BV2 microglia were pretreated with vehicle or naloxone for 30 minutes, followed by LPS treatment for 15 minutes. Membrane and cytosolic fractions were isolated to perform western blot analysis using p47^phox and gp91^phox antibodies. Results are expressed as a percentage of the control and are mean ± SEM of three independent experiments performed in duplicate. **P < 0.01 and ^## P < 0.01 compared with corresponding WT and LPS treatment group, respectively; LPS, lipopolysaccharide.