Figure 2
From: CCL2 upregulation triggers hypoxic preconditioning-induced protection from stroke
CCL2 protein expression in brain after HPC. (A) Temporal immunohistochemical analyses for CCL2 protein shows an early colocalization (at 6, 12, and 24 h) of CCL2 protein (red; middle panel) with NeuN-positive neurons (green; left panel) in the cortical grey matter after HPC (4 h, 8%O2). CCL2 reactivity was minimal in naïve animals and again at 2 days and 2 week after HPC. (B and C) Additional immunolabeling confirmed that CCL2 protein did not colocalize with (B) CD31-positive cortical endothelial cells (green; left panel), or with (C) GFAP-positive reactive astrocytes (green; left panel) within 24 h of HPC. ToPro3 (blue; right panel) was used in all photomicrographs as a general nuclear stain, and merged images are shown at the far right for each time point.