Retinal retinal ganglion cell (RGC) survival detected by Fluorogold (FG) retrograde labeling. (A) FG was transported retrograde to RGC soma in a whole-mount retina used as a control. From 7 to 21 days post-crush (7dPC to 21dPC, FG-labeled RGCs declined in (B) the WT and (C) trif
-/- groups. Scale bar, 100 μm. The number of labeled RGCs was analyzed to confirm that more RGCs survived in the trif
-/- group than in the WT group (D). *P < 0.05 vs. WT group. (E) Survival ratio of RGCs in trif
-/- group was higher than that in the WT group from 7dPC to 21dPC. *P < 0.05 vs. WT group.