Figure 3

OT-II regulatory T (T reg) cells reduce OT-I T cell-induced oligodendroglial (and collateral neuronal) apoptosis in acute brain slices form ODC-OVA mice. (A, B) Representative immunofluorescence images of the cortical gray matter of ODC-OVA slices incubated for 6 h with (A) or without (B) activated OT-I T cells. Apoptotic NogoA⁺ ODCs (left) and neuronal nuclear antigen (NeuN)⁺ neurons (right) were detected by staining for cleaved caspase-3. (C) Densities of cleaved caspase-3⁺ NogoA⁺ ODCs (left) and NeuN⁺ neurons (right) in slices from naïve ODC-OVA mice under the conditions indicated (n = 6, respectively). P values < 0.05 were considered significant (indicated as **). (D) Densities of cleaved caspase-3⁺ NogoA⁺ ODCs (left) and NeuN⁺ neurons (right) in slices from ODC-OVA mice treated intraperitoneally with lipopolysaccharide (LPS) (0.2 mg/kg) 24 h before preparation of brain slices under the conditions indicated (n = 5, respectively). P values < 0.05 were considered significant (indicated as **).