Figure 4

OT-II regulatory T (T reg) cells reduce release of cytotoxic effector molecules from OT-I T cells in acute brain slices form ODC-OVA mice. Representative flow cytometry analysis of activation markers (CD25, left panel) and intracellular effector molecules (granzyme B, right panel) of OT-I T cells retrieved and pooled from ODC-OVA slices incubated with activated OT-I T cells alone or together with OT-II T reg cells. Mean fluorescence intensities (MFI) of pooled OT-I T cells from slices incubated in the absence and presence of OT-II T reg cells were 455 vs 478 for CD25 (left panel) and 63 vs 79 for intracellular granzyme B (right panel).