Immunohistochemical localization of DPIV, DP8 and APN in contralateral cortices 7 days after eMCAO. Co-stainings were performed using the neuronal markers Neurotrace (A) or NeuN (F, J). DPIV (B), DP8 (E) and APN (I) -linked fluorescences were visualized by confocal laser scan microscopy. DAPI was used to stain cell nuclei (C, G, K, blue fluorescence). Furthermore, merged fluorescence images are shown (D, H, L). Contralateral hemispheres were not exposed to eMCAO. Note, DPIV immunostaining was not found under these conditions (see B). Similar results were obtained in untreated or sham-operated animals as well as 3 days after eMCAO in the contralateral cortex (not shown). Scale bars = 100 μm.