APN and cAAP protease activities after transient cerebral ischemia. (A) Relative Vmax of APN and cAAP activity in adult rat cortices of ipsilateral (infarct, black bars) or contralateral (control, gray bars) hemispheres at different time points after eMCAO. (B) Michaelis-Menten kinetics of total APN/cAAP activity in infarct (solid line) and control cortices (dashed line) at day 7 after eMCAO. Ipsilateral and contralateral cortices were characterized by roughly equal Km (infarct: Km = 160μM ± 0.004μM, n = 5; contralateral: Km = 160μM ± 0.019μM, n = 5) and similar Vmax values (infarct: 6.2 ± 2.1 nmol/min/mg protein, n = 5; contralateral: 5.5 ± 1.3 nmol/min/mg protein, n = 5). (C) Relative fractional contribution of APN (black) and cAAP (white) activity to total aminopeptidase activity (Ala-pNA hydrolysis) in the ipsilateral (infarct, left bar group) and contralateral (control, right bar group) hemisphere at different time points after eMCAO (pH 7.5).