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Figure 1 | Journal of Neuroinflammation

Figure 1

From: The cytokine tumor necrosis factor-like weak inducer of apoptosis and its receptor fibroblast growth factor-inducible 14 have a neuroprotective effect in the central nervous system

Figure 1

The interaction between TWEAK and Fn14 induces hypoxic tolerance. (A) Mean cell survival in Wt cerebral cortical neurons incubated 1 or 24 hours with TWEAK 100 ng/mL or 300 ng/mL. *P < 0.05 compared to cells incubated for 60 minutes with TWEAK 100 ng/mL. **P < 0.05 compared to cells incubated for 60 minutes with TWEAK 300 ng/mL. n = 15 per experimental group. Lines denote SD. (B) Mean cell survival in Wt cerebral cortical neurons exposed to 55 minutes of OGD conditions 24 hours after 60 minutes of incubation with TWEAK 0 to 300 ng/mL. n = 20 per experimental group; *, ** and *** P < 0.05 compared to neurons exposed to 55 minutes of OGD conditions without preconditioning. Lines denote SD. Values are given as cell survival compared to cells exposed to OGD conditions without preconditioning with TWEAK. (C) Wt cerebral cortical neurons were preconditioned with TWEAK for 60 minutes 24 hours before exposure to OGD conditions. Mean cell survival was determined with an MTT assay 24, 48 and 72 hours later. n = 12 per experimental group; *P < 0.05 compared to non-preconditioned neurons at each time point. Lines denote SD. (D) Mean cell survival in Wt and Fn14-/- cerebral cortical neurons exposed to 55 minutes of OGD conditions 24 hours after 60 minutes of incubation with TWEAK 100 ng/mL. n = 10; *P < 0.05 compared to Wt neurons non-preconditioned with TWEAK. Lines denote SD. Fn14: fibroblast growth factor-inducible 14; Ns: non-significant; OGD: oxygen-glucose deprivation; TWEAK: tumor necrosis factor-like weak inducer of apoptosis; Wt: wild-type.

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