Figure 2From: The cytokine tumor necrosis factor-like weak inducer of apoptosis and its receptor fibroblast growth factor-inducible 14 have a neuroprotective effect in the central nervous system Endogenous TWEAK mediates the neuroprotective effect of hypoxic preconditioning. (A) Mean fold increase in TWEAK and Fn14 mRNA expression in Wt cerebral cortical neurons 1, 3 and 6 hours after exposure to 30 minutes of OGD (sub-lethal hypoxia). n = 8, * and ** P < 0.05 compared to TWEAK mRNA expression in sister cultures maintained under normoxic conditions. ^, § and ¶ P < 0.05 compared to Fn14 expression in sister cultures maintained under normoxic conditions. Lines denote SD. (B-D) Mean cell survival in Wt (B), TWEAK-/- (C) and Fn14-/- (D) cerebral cortical neurons exposed to 55 minutes of OGD conditions (lethal hypoxia) 24 hours after 30 minutes of exposure to OGD conditions (hypoxic preconditioning, dark gray bars). A subgroup of neurons was exposed to lethal hypoxia without previous preconditioning (black bar). A sub-set of TWEAK-/- and Fn14-/- neurons was incubated with TWEAK 100 ng/mL during the preconditioning phase (hypoxic preconditioning, light gray bars). n = 12 per experimental group; * in B P < 0.05 compared to cells maintained under normoxic conditions; ** in B P <0.05 compared to neuronal cultures exposed to lethal hypoxia without preconditioning. * in C P < 0.05 compared to TWEAK-/- neurons either exposed to lethal OGD without previous preconditioning or preconditioned in the absence of TWEAK. Lines denote SD. Fn14: fibroblast growth factor-inducible 14; HPC: hypoxic preconditioning; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; Ns: non-significant; OGD: oxygen-glucose deprivation; TWEAK: tumor necrosis factor-like weak inducer of apoptosis.Back to article page