TLR4 mRNA and protein expression after ICH. A) Real-time RT-PCR shows an upregulation of TLR4 mRNA expression in perihematoma tissues in WT mice (n = 6) on days 1, 2, 3, and 5 post ICH. B) TLR4 co-labeling with tubulin-positive neuron (arrows, n = 6). C) TLR4 co-labeling with GFAP-positive astrocytes (arrows, n = 6). D) TLR4 co-labeling with CD11b-positive microglia. Compared to sham control mice (n = 6), ICH induced significant increase in TLR4 protein. *P < 0.05, **P < 0.01 vs. sham control. Values (mean ± SD) are representative of two independent experiments. Bar = 20 μM.