mice displayed attenuated brain injury and decreased neuroinflammation. TLR4−/− had decreased brain water content (A, n = 3) and decreased NDS (B, n = 3). ELISA showed that TLR4−/− mice had a marked decrease in release of IL-6, TNF-α, and IL-1β (C, n = 3) on day 3 post-ICH. D) Immunohistochemistry of CD68 showed decreased macrophage infiltration in TLR4−/− mice on day 3 post-ICH (n = 3). **P < 0.01 vs. sham group; ##
P < 0. 01 vs. WT group; Bar = 50 μM in D. Values (mean ± SD) are representative of three independent experiments.