mice showed reduced MyD88 and TRIF protein level and NF-κB activity. Western blot showed TLR4−/− had decreased expression of MyD88 (A) and TRIF (B). Electrophoretic mobility shift assay showed decreased NF-κB activity in TLR4−/− (C) **P < 0.01 vs. sham group, ##
P < 0. 01 vs. WT group. Values (mean ± SD, n = 3 for each group) are representative of three independent experiments.