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Figure 12 | Journal of Neuroinflammation

Figure 12

From: NADPH oxidase and reactive oxygen species contribute to alcohol-induced microglial activation and neurodegeneration

Figure 12

DPI reduced ethanol-induced caspase-3 activation and Fluoro-Jade B staining. Male C57BL/6 mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days. DPI (3 mg/kg) was injected intraperitoneally 0.5 hr and 24 hr after the last dose of ethanol. Mice were sacrificed 3 hrs after the last dose of DPI. Brain sections were stained with polyclonal cleave caspase-3 antibody and Fluoro-Jade B, respectively. (A) Ethanol increased the number of caspase-3+IR cells. DPI significantly reduced ethanol-induced increases in the number of caspase-3+IR cells. (B) Fluorescent intensity of Fluoro-Jade B positive cells in cortex was quantified by BioQuant image analysis system. Ethanol-treated mouse brains showed more Fluoro-Jade B positive cells than water-treated mouse brains. DPI significantly decreased ethanol-induced increases in the fluorescent intensity of Fluoro-Jade B positive cells. ** P < 0.01, compared with water controls. ## P < 0.01, compared with ethanol-treated group.

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