Skip to main content
Figure 1 | Journal of Neuroinflammation

Figure 1

From: NADPH oxidase and reactive oxygen species contribute to alcohol-induced microglial activation and neurodegeneration

Figure 1

Chronic ethanol increases caspase-3 activation. (A) Representative images of activated caspase-3+immunoreactive (+IR) cells in cortex and dentate gyrus (DG) from control and ethanol-treated mice. (B) Quantification of caspase-3 immunoreactivity in cortex and DG. Ethanol-treated mouse brains showed more caspase-3+IR cells than water controls 24 hrs after the last dose of ethanol treatment. (C) Brain sections from water and ethanol (5 g/kg, i.g., 10 days) treated C57BL/6 mice were double-stained with cleaved caspase-3 and Neu-N (a neuronal marker) antibodies. Shown are images of cortex and DG for activated caspase-3 (green), Neu-N (red), and colabeling of caspase-3 and Neu-N (yellow). Confocal microscopy shows that caspase-3 is expressed in Neu-N+IR cells, as shown with arrows indicating the colabeling of caspase-3 and Neu-N in ethanol-treated mice. Control sections show little or not colabeling of caspace-3 and Neu-N+IR cells. Inset is the higher magnification of arrow pointed cells. ** P < 0.01, compared with the water control mice (n = 10). Scale bar = 30 μm; inset 5 μm.

Back to article page