Figure 3

Increased Fluoro-Jade B staining in human post-mortem alcoholic brain (A) Representative images from the orbitofrontal cortex (OFC) of moderate drinking control brain and alcoholic brain stained with Fluoro-Jade B. Brain sections from the OFC of human post-mortem alcoholics and moderate drinkers were double-labeled with Neu-N antibody and Fluoro-Jade B. Confocal microscopy indicated that Fluoro-Jade B positive cells were colocalized with Neu-N, suggesting neuronal death Scale bar = 30 μm. (B) Level of fluorescent intensity of Fluoro-Jade B positive cells was quantified by BioQuant image analysis software. The OFC of human post-mortem alcoholic brain had significant increase in fluorescent intensity of Fluoro-Jade B positive cells, compared to the OFC of human moderate drinking control brain (** P < 0.01, n = 8).