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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Inflammatory monocytes damage the hippocampus during acute picornavirus infection of the brain

Figure 4

Characterization of the Ly6 antigen series on brain-infiltrating leukocytes (BILs) and peripheral blood leukocytes (PBLs). Flow cytometric analysis of PBLs (A, C, E, G, I, K, M, O) and BILs (B, D, F, H, J, L, N, P) collected from the same mouse 18 h after infection with Theiler's murine encephalomyelitis virus (TMEV) revealed the presence of readily distinguishable neutrophil populations in both cell preparations. However, inflammatory monocytes were only detected in the BILs. Consistent with the analyses performed above, the CD45hiCD11b++F4/80+ population of inflammatory monocytes was positive for Gr1 (Ly6C/G), 7/4 (Ly6B), and AL-21 (Ly6C) but negative for 1A8 (Ly6G). The CD45hiCD11b+++F4/80- neutrophil population was positive for all four Ly6 series antigens. Of note, CD45hiCD11b++F4/80+ inflammatory monocytes in the BILs expressed higher levels of surface Ly6C/G (B, D) and Ly6B (J, L) as compared to CD45hiCD11b+++F4/80- neutrophils and expressed 10-fold higher levels of the AL-21 antigen (N, P). The CD45+CD11b+ macrophage population in the blood was negative for all Ly6 series antigens. Results are representative of five mice.

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