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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Inflammatory monocytes damage the hippocampus during acute picornavirus infection of the brain

Figure 6

Visualization of the green flourescent protein (GFP) + infiltrate in the brain of Theiler's murine encephalomyelitis virus (TMEV)-infected LysM:eGFP mice. TMEV-infected LysM:eGFP reporter mice were killed by intracardiac perfusion of 4% paraformaldehyde 18 h after infection. Vibratome sections were prepared and analyzed by epifluorescence microscopy. The distribution of GFP+ neutrophils and inflammatory monocytes at low magnification (A, B) matches the distribution of inflammatory infiltrate observed in Figure 1. Higher magnification of a cross-section through the alveus and corpus callosum (C) and at the hippocampal fissure (D) shows the presence of both GFPhi and GFPmid cells with a variety of morphologies consistent with migratory behavior. Comparison of the epifluorescent signal (E) and the brightfield image (F) of a cross-section of the hippocampus confirms the presence of numerous GFP+ neutrophils and inflammatory monocytes at the hippocampal fissure (HF), in the alveus (alv), and proximal to the pyramidal neurons of CA1. Cg = cingulum bundle; DG = dentate gyrus. Green represents GFP in (A-E), blue in (C-D) shows 4',6-diamidino-2-phenylindole (DAPI). Results are representative of eight mice.

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