Methamphetamine increases the expression of IL-6 and IL-8 in astrocytes. 0.8 × 106 SVGA astrocytes were treated with different doses of MA for 24 hours and total mRNA was isolated. The relative expression levels of IL-6 (A) and IL-8 (B) were measured using real time RT-PCR as compared to untreated control. 0.8 × 106 SVGA were treated with 500 μM of MA for three days in flasks and the cells were harvested 24 hours after the last dose. Total mRNA was isolated and the relative levels of IL-6 (C) and IL-8 (D) were determined using real time RT-PCR as compared with untreated control. (E) Primer sequences used in order to measure the mRNA expression of IL-6 and IL-8 using real time RT-PCR. Each bar represents the mean ± SE of three experiments with each experiment performed in triplicate. The statistical significance was calculated using student's t-test and ** denotes P ≤0.01. MA, methamphetamine.