Leukotrienes (LTs) reduce susceptibility of monocyte-derived microglial-like cells (MDMis) to HIV-1 infection. (A) MDMis were pretreated for 45 minutes with 100 ng/ml of the indicated LTs prior to infection with YU2. At 2 h after infection, cells were then thoroughly washed and incubated in fresh media containing the listed LTs. Additional LTs were also replenished at days 3 and 6 post infection. Viral production was monitored at 3, 6 and 9 days post infection by measuring the p24 content. The results shown are from one representative donor of a total of three. (B) The p24 values for all three donors tested are depicted as percentage of untreated control to compensate for donor-to-donor virus infection variability. (C) Production of infectious virus particles by LT-treated MDMis was quantified at 9 days post infection in cell-free supernatants using the TZM-bl indicator cell line. Results shown represent mean luciferase activity ± SD calculated from three independent experiments with triplicate samples and are expressed as the relative percentage of infectious virus production with respect to non-treated cells (*P < 0.05).