Figure 4
From: Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells
Leukotriene (LT) receptor antagonists block the modulatory effect of LTB 4 and LTC 4 on HIV-1 replication in monocyte-derived microglial-like cells (MDMis). Cells were either left untreated (Ctrl) or treated with the listed LT receptor antagonists (that is, LY293111 or Bay-u9773) for 45 minutes before being thoroughly washed. MDMis were then either exposed to LTs 45 minutes before or 2 h following HIV-1 infection, after which cells were thoroughly washed. Virus infection was carried out using JR-FL pseudotyped viruses. The extent of virus infection was estimated by measuring virus-encoded luciferase activity at 7 days post infection. The results shown represent the means ± SD calculated from three independent experiments with triplicate samples and are expressed as the percentage of luciferase activity with respect to non-treated cells (*P < 0.05).