Figure 9

Effect of Krüppel-like factor 4 overexpression on the anti-inflammatory properties of honokiol. Total cellular extract isolated from cells transfected with K-10 and LacZ constructs and treated with 500 ng/mL of LPS along with different doses of HNK, analyzed by immunoblotting for Klf4, iNOS and Cox-2. (A) Expression levels of Klf4 in different conditions showing increased expression of Klf4 in LPS-treated with respect to untreated control as well as a significant increase in Klf4 in K-10 + LPS cells with respect to LPS alone condition. (B) Cox-2 expression showing increased expression under K-10 + LPS as well as HNK-treated K-10 + LPS conditions with respect to LPS alone or control untreated condition. (C) Expression levels of iNOS showing significant increase in its levels in K-10 + LPS and K-10 + LPS conditions treated with HNK compared to LPS alone-treated condition. Graphs represent fold change in protein levels with respect to untreated control condition. Data represent mean ± SEM from three independent experiments performed in duplicate. **P < 0.01 in comparison to LPS-treated values.