Figure 8

Oxidative stress markers are only present in Purkinje cells. (A) End products of lipid peroxidation, predominantly malondialdehyde, were quantified by the TBARS assay. No differences between 9- to 12-week-old Nestin-Pex5 ^−/− and control littermates were observed in the corpus callosum, cerebellum or spinal cord. Values are given as means ± SEM of four independent samples of each genotype (Student’s t-test). (B-I) The markers 4-HNE (B-E) and 3-nitrotyrosine (3-NT) (F-I) were evaluated by immunohistochemistry. 4-HNE was slightly observable in Purkinje cells of two-week-old Nestin-Pex5 ^−/− mice (C), compared with the control littermates (B). The amount of 4-HNE present in Purkinje cells was higher at three (D) and 12 weeks (E). Low 3-NT immunoreactivity was observed at three weeks in Purkinje cells (G), but increased with age (H-I). Scale bars: B-F and G: 100 μm; G and I: 50 μm. 4-HNE, 4-hydroxynonenal; SEM, standard error of the mean; TBARS, thiobarbituric acid reactive substances.