Clone 10 specificity and binding efficiency in mouse tissue. Using fluorescence-activated cell sorting (FACS) analysis (A), mouse astrocytes identified by glial fibrillary acidic protein (GFAP) were examined for extracellular matrix metalloproteinase inducer (EMMPRIN) expression using hybridoma supernatants from several clones (1 to 13, no clone 5) and commercial anti-mouse EMMPRIN antibody (e-Bioscience). Tissues from E15 embryos were used to determine genotype by PCR (B), where a band at 285 bp identified the PCR product for neo primers (knockout (KO) band), and a band at 503 bp identified the PCR product for EMMPRIN wild-type (WT); these tissues were subjected to FACS analysis (C) for EMMPRIN WT, heterozygous (Het) and KO mice. (D) EMMPRIN WT, Het and KO tissues were used in immunofluorescence staining with clone 10, IgM isotype control or commercial anti-EMMPRIN antibody (Anti-EMMPRIN; Serotec). Importantly, clone 10 and the Serotec antibody did not stain EMMPRIN KO tissue.