Figure 7

Treatment with clone 10 attenuates experimental autoimmune encephalomyelitis (EAE) disease severity. (A) Mice immunized for EAE were treated with 50 μg/mouse of clone 10 (red circles) or IgM isotype control (Black squares) at days 8, 11 and 15 (arrows) post-myelin oligodendrocyte glycoprotein (MOG) immunization. EAE disease severity was found to be significantly reduced in clone 10 treated animals compared with mice treated with an IgM isotype control. The data points have an overall statistical difference between timepoints 10 and 16 (*P = 0.04, Mann Whitney U test). Results are mean ± SEM from five mice each; this trend was reproduced in another experiment. (B) Spinal cord sections from EAE animals treated with clone 10 or IgM isotype control and killed at day 16 were stained with hematoxylin and eosin and Luxol Fast Blue to determine sites of immune cell infiltration and myelin sheaths. Images were captured at 10 × and 20 × original magnification.