Table 1 List of primary antibodies used for immunoblotting (IB), immunohistochemistry (IHC), and immunocytochemistry (ICC)
Primary antibodies | Clone # | Host | Company | Catalog # | Application | Folds |
|---|---|---|---|---|---|---|
Arginase-1 | 19/Arginase I | Mouse | BD Pharmingen (Franklin Lakes, NJ) | 61708 | WB | 2,000 |
β-Actin | AC-74 | Mouse | Sigma (St Louise, MO) | A5316 | WB | 4,000 |
CD11b | 5C6 | Rat | Serotec (Oxford, UK) | MCA711 | ICC | 250 |
Cyclooxygenase 2 COX2) | Â | Rabbit | Cayman Chemical (Ann Arbor, MI) | 160106 | WB | 8,000 |
F4/80 | CI:A3-1 | Rat | BMA Biomedicals (Augst, Swizerland) | T-2008 | IHC | 500 |
GAPDH | 6C5 | Mouse | Chemicon International (Temecula, CA) | MAB374 | WB | 4000 |
Glial fibrially acidic protein (GFAP) | G-A-5 | Mouse | Sigma (St Louise, MO) | G3893 | ICC | 250 |
| Â | Â | Rabbit | DAKO (Glostrup, Denmark) | N1506 | IHC | 10 |
Iba-1 | Â | Rabbit | WAKO (Osaka, Japan) | 019-19741 | IHC | 500 |
Insulin-like growth factor 1 | Sm1.2 | Mouse | Upstate (Lake Placid, NY) | 05-172 | IHC | 100 |
(IGF-1) | Â | Â | Â | Â | Â | Â |
Interleukin-1β (IL-1β) |  | Goat | R & D systems (Minneapolis, MN) | AF-401-NA | IHC | 100 |
CD206 | MR5D3 | Rat | AbD Serotec (Raleigh, NC)) | MCA2235GA | WB | 4,000 |
myelin basic protein (MBP) | Â | Rat | Chemicon International (Temecula, CA) | MAB386 | ICC | 250 |
NeuN | Â | Mouse | Chemicon International (Temecula, CA) | MAB377 | ICC | 250 |
Neuron specific enolase (NSE) | 3-(3)-C | Mouse | IBI (Fujioka, Japan) | 11031S | IHC | 1,000 |
Inducible nitric oxide synthase (iNOS/NOS type II) | Â | Rabbit | Transduction Laboratories (Lexington, KY) | N32030 | WB | 10,000 |
STAT1 | 42 | Mouse | Transduction Laboratories (Lexington, KY) | S21120 | WB | 5,000 |
Ym1 | Â | Rabbit | StemCell Tech (Vancouver, BC, Canada) | 01404 | WB | 1,500 |