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Table 1 List of primary antibodies used for immunoblotting (IB), immunohistochemistry (IHC), and immunocytochemistry (ICC)

From: Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury

Primary antibodies Clone # Host Company Catalog # Application Folds
Arginase-1 19/Arginase I Mouse BD Pharmingen (Franklin Lakes, NJ) 61708 WB 2,000
β-Actin AC-74 Mouse Sigma (St Louise, MO) A5316 WB 4,000
CD11b 5C6 Rat Serotec (Oxford, UK) MCA711 ICC 250
Cyclooxygenase 2 COX2)   Rabbit Cayman Chemical (Ann Arbor, MI) 160106 WB 8,000
F4/80 CI:A3-1 Rat BMA Biomedicals (Augst, Swizerland) T-2008 IHC 500
GAPDH 6C5 Mouse Chemicon International (Temecula, CA) MAB374 WB 4000
Glial fibrially acidic protein (GFAP) G-A-5 Mouse Sigma (St Louise, MO) G3893 ICC 250
   Rabbit DAKO (Glostrup, Denmark) N1506 IHC 10
Iba-1   Rabbit WAKO (Osaka, Japan) 019-19741 IHC 500
Insulin-like growth factor 1 Sm1.2 Mouse Upstate (Lake Placid, NY) 05-172 IHC 100
(IGF-1)       
Interleukin-1β (IL-1β)   Goat R & D systems (Minneapolis, MN) AF-401-NA IHC 100
CD206 MR5D3 Rat AbD Serotec (Raleigh, NC)) MCA2235GA WB 4,000
myelin basic protein (MBP)   Rat Chemicon International (Temecula, CA) MAB386 ICC 250
NeuN   Mouse Chemicon International (Temecula, CA) MAB377 ICC 250
Neuron specific enolase (NSE) 3-(3)-C Mouse IBI (Fujioka, Japan) 11031S IHC 1,000
Inducible nitric oxide synthase (iNOS/NOS type II)   Rabbit Transduction Laboratories (Lexington, KY) N32030 WB 10,000
STAT1 42 Mouse Transduction Laboratories (Lexington, KY) S21120 WB 5,000
Ym1   Rabbit StemCell Tech (Vancouver, BC, Canada) 01404 WB 1,500